Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-5124a

Tough Decoy (TuD) Design

제품 스펙

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm employs the Tough Decoy (TuD) design, known for effective inhibition of target miRNAs.

miRNAs regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector with compatible packaging plasmids produces viral particles suitable for transducing mammalian cells.

The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) to enhance transgene expression and carries a puromycin resistance gene for selection.

주요 특징

• 강력한 miRNA 억제 효과 제공
• 렌티바이러스 전달 형식을 통한 다양한 세포 유형에서의 효율적 전달
• 재형질감염 없이 장기적 억제 가능
• 퓨로마이신 내성 유전자 포함으로 선택 용이