Merck PSF-MINCMV-ZEO - MINIMAL CMV ZEOCIN RESISTANT PLASMID

PSF-MINCMV-ZEO - MINIMAL CMV ZEOCIN RESISTANT PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4105 bp

박테리아 선정

kanamycin

포유류 세포 선정

Zeocin®

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

PSF-MINCMV-ZEO - MINIMAL CMV ZEOCIN RESISTANT PLASMID has the Zeocin resistance gene under transcriptional control of the minimal CMV promoter giving basal expression in most mammalian cells. Expression levels can be influenced by inserting additional regulatory elements upstream of the minimal promoter using a specially-positioned MCS for example to provide dependence on cell-associated transcription factors or externally applied drugs such as doxycycline. In this way a range of sophisticated constructs can be prepared that endow Zeocin resistance only under specific conditions.

Promoter Expression Level: This plasmid vector contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.