Merck PSF-MINCMV-BLAST - BLASTICIDIN SELECTION MINIMAL CMV VECTOR

PSF-MINCMV-BLAST - BLASTICIDIN SELECTION MINIMAL CMV VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4159 bp

박테리아 선정

kanamycin

포유류 세포 선정

blasticidin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

Here the blasticidin resistance gene is under transcriptional regulation of the minimal CMV promoter giving only low level expression in most mammalian cells. Expression of the resistance gene can be regulated by inserting additional regulatory components upstream of the promoter using a specially-positioned MCS for example sites that respond to specific transcription factors or to externally-applied drugs such as doxycycline. In this way sophisticated constructs can be prepared that provide blasticidin resistance only under certain pre-defined conditions.

Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.