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PSF-TEFI-URA3 - URACIL YEAST SELECTION PLASMID
plasmid vector for molecular cloning
expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid
form
buffered aqueous solution
분자량
size 6720 bp
박테리아 선정
kanamycin
복제개시점
2Micron
pUC (500 copies)
펩타이드 절단
no cleavage
프로모터
Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast
리포터 유전자
none
배송 상태
ambient
저장 온도
−20°C
효모 선정
uracil
PSF-TEFI-URA3 – uracil yeast selection plasmid is a yeast expression plasmid vector containing the yeast EF1a strong constitutive promoter and the URA3 selection marker to allow growth in the absence of uracil. PSF-TEFI-URA3 – uracil yeast selection expression plasmid is designed for the production proteins in Saccharomyces cerevisiae with selection on media that is deficient in the metabolite uracil. The vector contains the constitutive TEF1 yeast promoter to drive the expression of a gene of interest. It also contains a gene that is an essential component of the uracil synthesis pathway. This allows the plasmid to be maintained in yeast cells that have this gene deleted on media that does not contain uracil. This is the most commonly used selection method for Saccharomyces cerevisiae. We also provide other metabolite selection yeast plasmids that use histidine leucine or tryptophan as the selection method. We also provide plasmids using small molecule selection such as puromycin and blasticidin.
Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae.
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