Merck PSF-TEFI-URA3 - URACIL YEAST SELECTION PLASMID

PSF-TEFI-URA3 - URACIL YEAST SELECTION PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 6720 bp

박테리아 선정

kanamycin

복제개시점

2Micron
pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

효모 선정

uracil

PSF-TEFI-URA3 – uracil yeast selection plasmid is a yeast expression plasmid vector containing the yeast EF1a strong constitutive promoter and the URA3 selection marker to allow growth in the absence of uracil. PSF-TEFI-URA3 – uracil yeast selection expression plasmid is designed for the production proteins in Saccharomyces cerevisiae with selection on media that is deficient in the metabolite uracil. The vector contains the constitutive TEF1 yeast promoter to drive the expression of a gene of interest. It also contains a gene that is an essential component of the uracil synthesis pathway. This allows the plasmid to be maintained in yeast cells that have this gene deleted on media that does not contain uracil. This is the most commonly used selection method for Saccharomyces cerevisiae. We also provide other metabolite selection yeast plasmids that use histidine leucine or tryptophan as the selection method. We also provide plasmids using small molecule selection such as puromycin and blasticidin.

Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae.