Merck PSF-STE5-URA3 - WEAK PROMOTER YEAST PLASMID

PSF-STE5-URA3 - WEAK PROMOTER YEAST PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 6519 bp

박테리아 선정

kanamycin

복제개시점

2Micron
pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

효모 선정

uracil

This plasmid is designed for low level expression of proteins in Saccharomyces cerevisiae using the STE5 promoter. This is the weakest of the promoters that we sell for expression in yeast. The plasmid also contains a gene that is an essential component of the uracil synthesis pathway. This allows for the selection of yeast cells that are defective in this gene in media that does not contain uracil.

Promoter Expression Level: This plasmid contains the yeast scaffold protein STE5 promoter. It is the weakest promoter that we provide for expression in Saccharomyces cerevisiae.