Merck PSF-CMV-HULAMBDA LC - HUMAN LAMBDA LIGHT CHAIN ANTIBODY VECTOR

PSF-CMV-HULAMBDA LC - HUMAN LAMBDA LIGHT CHAIN ANTIBODY VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4576 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

An antibody expression plasmid designed for the production of human antibody lambda genes by fusing variable antibody fragments with the constant domain in this plasmid. This can be achieved because the contstant region coding sequence in this plasmid is positioned downstream of a BseRI restriction site which when cleaved will produce an overhang consisting of the first two nucleotides of the first codon of the constant region. This allows variable antibody fragments to be seamlessly inserted by placing a BseRI site in the opposing direction at the end of the variable fragment. We also provide plasmids for the expression of both rat and mouse antibodies as well as vectors for the expression of human IgG heavy chain and Kappa light chain (called pSF-CMV-HuIgG1 HC and pSF-CMV-HuKappa LC respectively)

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.