Merck PSF-CMV-ILUMENA - MAMMALIAN SECRETED LUCIFERASE PLASMID

PSF-CMV-ILUMENA - MAMMALIAN SECRETED LUCIFERASE PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4919 bp

박테리아 선정

ampicillin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

iLumena luciferase

배송 상태

ambient

저장 온도

−20°C

A plasmid designed for the expression of secreted luciferase in mammalian cells. The iLumena reporter gene in this vector has been specifically engineered for expression in mammalian cells and yields high levels of luminsceence in the supernatant. The substrate for the luciferase reporter is coelenterazine.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. The ubiquitin promoter driving reporter gene expression is the strongest endogenous human promoter that we provide for most cell types.