Merck PSF-CMV-RAT-KAPPA LC - RAT KAPPA LIGHT CHAIN ANTIBODY PLASMID

PSF-CMV-RAT-KAPPA LC - RAT KAPPA LIGHT CHAIN ANTIBODY PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4576 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This vector is for the production of kappa antibody light chains for rat (Rattus rattus). It has been engineered so that when the vector is cut with the restriction enzyme BseRI it produces an overhang from the first codon of the constant region. This allows antibody light chain variable regions to be seamlessly ligated into this vector to create full length rat kappa light chain antibody expression cassettes. As part of this vector range we also provide plasmids for the production of both human and mouse antibodies as well as vectors for the creation of Heavy IgG chain and Lambda light chain rat antibodies (named pSF-CMV-Rat-IgG HC and pSF-CMV-Rat-Lambda respectively)

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.