Merck PSF-CMV-RAT-IGG1 HC - RAT IGG1 HEAVY CHAIN ANTIBODY PLASMID

PSF-CMV-RAT-IGG1 HC - RAT IGG1 HEAVY CHAIN ANTIBODY PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 5236 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This plasmid is designed for the generation and expression of heavy chain rat (Rattus rattus) immunoglobulin antibody genes. The vector has been formatted so that it can be cleaved to produce a restriction site overhang (using BseRI) within the first codon of the antibody constant region. This allows the variable regions of antibodies to be inserted to create full length heavy chain expression cassettes. As part of this product range we also have antibody vectors for the production of mouse and human antibodies as well as plasmids for the production of rat Kappa and Lambda antibody light chains (pSF-CMV-Rat-Kappa and pSF-CMV-Rat-Lambda respectively).

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.