Merck PSF-TAC - PTAC INDUCIBLE BACTERIAL PLASMID

PSF-TAC - PTAC INDUCIBLE BACTERIAL PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 3864 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: Tac
Promoter activity: inducible
Promoter type: bacterial

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This is a bacterial expression plasmid containing pTAC promoter that can be regulated using the Lac repressor. The pTac promoter was created by fusing the promoter for the Lac operon with the promoter for the Tryptophan operon. This provides greater control and expression in comparison to standard Lac promoter plasmids. In order to use this plasmid you will need an E. coli cell line that expresses the LacI repressor this is a common feature of most cloning and protein expression E. coli strains.

Promoter Expression Level: This plasmid contains the IPTG inducible promoter that was created by the fusion of the Lac promoter and the Tryptophan operon promoter. It allows inducible expression in E. coli using IPTG as the inducing agent.