Merck Tris-HCl Buffer, pH 10, 10×, Antigen Retriever

Tris-HCl Buffer, pH 10, 10×, Antigen Retriever

Quality Level

100

생물학적 소스

synthetic

pH

10

application(s)

hematology
histology

저장 온도

2-8°C

Tris-HCl Buffer, pH 10, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Tris-HCl buffer, pH 10, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.