Servicebio Anti -beta Actin Mouse mAb
Servicebio의 Anti-beta Actin Mouse mAb은 세포골격 단백질 β-Actin을 검출하는 고품질 항체로, WB·IHC·IF 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며 인간, 마우스, 랫트 시료에 모두 사용 가능합니다. 연구용으로만 제공됩니다.
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Product Information
| 항목 | 내용 |
|---|---|
| Protein full name | Actin, cytoplasmic 1 |
| Synonyms | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin |
| Immunogen | KLH conjugated synthetic peptide corresponding to Mouse β-Actin |
| Uniprot ID | P60710 |
| Isotype | IgG1, κ |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm |
| Predicted MW / Observed MW | 42 kDa / 45 kDa |
Applications
| Application | Species | Dilution Range | Sample Type |
|---|---|---|---|
| WB | Human, Mouse, Rat | 1:1000–1:2000 | Spleen, Kidney, Brain |
| IHC/IF | Human, Mouse, Rat | 1:400–1:2000 | Colon, Kidney, Liver |
| ICC/IF | Human, Mouse, Rat | 1:300–1:1000 | NIH/3T3, A549 |
Background
Actins are highly conserved proteins involved in various types of cell motility and are expressed in all eukaryotic cells. In vertebrates, three main actin isoform groups—alpha, beta, and gamma—have been identified. Alpha actins are found in muscle tissues and form the contractile apparatus, while beta and gamma actins coexist in most cell types as cytoskeletal components and mediators of internal cell motility.
Western Blot Results (OCR Extracted Data)
Image 1 (Beta Actin WB Analysis):
60 kDa —
45 kDa —
35 kDa —
HeLa
HepG2
MCF-7
Mouse spleen
Mouse kidney
Mouse brain
Rat kidney
Rat brain
beta ActinImage 2 (Beta Actin WB Analysis):
55 kDa —
43 kDa —
34 kDa —
THP-1 MCF-7 Lncap NIH/3T3 RAW 264.7 PC-12 Mouse brain Rat brain
beta ActinStorage
| 항목 | 내용 |
|---|---|
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA, and 50% glycerol. |
Notes
- This product is intended for research use only.
- Recommended to dilute with Primary Antibody Dilution Buffer (G2025).
Related Product Classification
- Antibodies
- Primary Antibodies
- Loading Control Antibodies
Servicebio is one of the leading manufacturers in China providing FDA & CE approved lab supplies and services.
References (Selected Publications Using This Antibody)
- Xu, Y., et al. ACS Nano 14, 9780–9795 (2020). PMID: 32915543 — Application: IHCIF, IF 17.1
- Wu, Y., et al. Biomaterials 279, 121237 (2021). PMID: 34749071 — Application: WBIF 14
- Liu, L., et al. J Hazard Mater 438, 129550 (2022). PMID: 35999725 — Application: WBIF 13.6
- Zhou, Y.-S., et al. Phytomedicine 96, 153910 (2022). PMID: 35026502 — Application: WBIF 7.9
- Tong, L., et al. Nutrients 13, 3319 (2021). PMID: 34684320 — Application: WBIF 6.706
(Additional references available upon request)
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