Servicebio Anti -beta Actin Mouse mAb

Product Information

Protein full name	Actin, cytoplasmic 1
Synonyms	ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse β-Actin
Uniprot ID	P60710
Isotype	IgG1,κ
Purity	Affinity purification
Subcellular location	Cytoplasm
Predicted MW. / Observed MW.	42 kDa / 45 kDa

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Applications

WB	Human, Mouse, Rat	1: 1000-1: 2000	spleen, kidney, brain
IHC/IF	Human, Mouse, Rat	1: 400-1: 2000	colon, kidney, liver
ICC/IF	Human, Mouse, Rat	1: 300-1: 1000	NIH/3T3, A549

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Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

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Images

	Western blot analysis of beta Actin (GB12001) at dilution of 1: 1000
	Western blot analysis of beta Actin (GB12001) at dilution of 1: 1000
	Immunohistochemistry of paraffin embedded human colon using actin (GB12001) at dilution of 1: 1000 (200x lens)
	Immunohistochemistry of paraffin embedded rat liver using actin (GB12001) at dilution of 1: 1000 (200x lens)
	Immunohistochemistry of paraffin embedded mouse stomach using actin (GB12001) at dilution of 1: 1000 (400x lens)
	Immunohistochemistry of paraffin embedded rat kidney using actin (GB12001) at dilution of 1: 1000 (400x lens)
	Immunohistochemistry of paraffin embedded human liver cancer using actin (GB12001) at dilution of 1: 1000 (200x lens)
	Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB12001) at dilution of 1: 1000
	Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using β-Actin (GB12001) at dilution of 1: 1000

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Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

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NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Classification of related products

Products

Antibodies

Primary Antibodies

Loading Control Antibodies

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