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1 / 2Servicebio Anti -GAPDH Rabbit pAb
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GAPDH 단백질을 인식하는 토끼 폴리클로날 항체로, WB와 IHC/IF에 적합합니다. 인간, 생쥐, 랫드 시료에서 높은 특이성과 재현성을 제공합니다. 고순도 IgG로 정제되었으며, -20℃ 보관 시 안정적입니다.
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Servicebio GB11002 Anti-GAPDH Rabbit pAb, 100ul (No.2/132) pk판매 단위 pk ·
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Servicebio · Servicebio Anti -GAPDH Rabbit pAb
Product Information
| 항목 | 내용 |
|---|---|
| Protein full name | Glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms | GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
| Immunogen | KLH conjugated synthetic peptide corresponding to Mouse GAPDH |
| Uniprot ID | P16858 |
| Isotype | IgG |
| Purity | Affinity purification |
| Predicted MW / Observed MW | 36 kDa / 36 kDa |
Applications
| Application | Species | Dilution | Sample |
|---|---|---|---|
| WB | Human, Mouse, Rat | 1:1000–1:2000 | brain, spleen, kidney |
| IHC/IF | Human, Mouse, Rat | 1:500–1:1000 | kidney, testis |
Background
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme (~37 kDa) catalyzing the sixth step of glycolysis, facilitating glucose breakdown for energy and carbon molecules. Beyond its metabolic role, GAPDH is involved in transcription activation, apoptosis initiation, ER-Golgi vesicle transport, and axonal transport. In sperm, a testis-specific isoenzyme (GAPDHS) is expressed.
Western Blot Analysis (OCR Text)
Image 1 (WB):
HeLa HepG2 MCF-7 Mouse liver Mouse kidney Mouse brain Rat liver Rat kidney Rat brain
45 kDa—
35 kDa—
25 kDa—
GAPDH
Image 2 (WB):
43 kDa—
34 kDa—
26 kDa—
HeLa HepG2 MCF7 Mouse brain Mouse spleen Rat kidney Rat brain
GAPDH
Storage
| 항목 | 내용 |
|---|---|
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
Notes
- This product is intended for research use only.
- Recommended to dilute with Primary Antibody Dilution Buffer (G2025).
Related Publications
- Jia, Y. et al. Molecular Cancer 20, 162 (2021). PMID 34893064 — Application: WB/IF
- Yao, Y. et al. Nature Biomedical Engineering 6, 1257–1271 (2022). PMID 36217021 — Application: WB/IF
- Ju, W. et al. Journal of Biomedical Science 27, 91 (2020). PMID 32873283 — Application: WB/IF
- Hong, N. et al. Cell Death & Disease 12, 393 (2021). PMID 33846290 — Application: IF
- Bian, H. et al. Journal of Neuroinflammation 17, 166 (2020). PMID 32450903 — Application: WB/IF
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