Servicebio Anti -GAPDH Mouse mAb
GAPDH 단백질을 인식하는 Mouse monoclonal antibody로, Western blot과 IHC/IF에 적합합니다. Human, Mouse, Rat 시료에서 사용 가능하며, 고순도 Affinity 정제 제품입니다. 세포 내 대사 및 비대사 기능 연구에 활용됩니다.
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Product Information
| 항목 | 내용 |
|---|---|
| Protein full name | Glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms | GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
| Immunogen | KLH conjugated synthetic peptide corresponding to Mouse GAPDH |
| Uniprot ID | P16858 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm, Nucleus, Membrane |
| Predicted MW / Observed MW | 36 kDa / 36 kDa |
Applications
| Application | Species | Dilution | Sample |
|---|---|---|---|
| WB | Human, Mouse, Rat | 1:500–1:1000 | spleen, lung, kidney |
| IHC/IF | Human, Mouse, Rat | 1:200–1:400 | kidney, stomach, testis |
Background
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme of approximately 37 kDa that catalyzes the sixth step of glycolysis, breaking down glucose for energy and carbon molecules. Beyond its metabolic role, GAPDH participates in transcription activation, apoptosis initiation, ER-to-Golgi vesicle transport, and axonal transport. In sperm, a testis-specific isoenzyme GAPDHS is expressed.
Experimental Data (OCR Extracted)
WB Analysis 1:
HeLa, HepG2, MCF-7, Mouse spleen, Mouse lung, Mouse kidney, Rat spleen, Rat lung, Rat kidney
43 kDa —
34 kDa —
GAPDH
WB Analysis 2:
HepG2, NIH/3T3, PC-12, Mouse skeletal muscle, Mouse brain, Rat heart, Rat skeletal muscle, Rat brain
43 kDa —
34 kDa —
GAPDH
Storage
| 항목 | 내용 |
|---|---|
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA, and 50% glycerol. |
Note
- This product is intended for research use only.
- Recommended dilution buffer: Primary Antibody Dilution Buffer (G2025).
Related Publications (Selected)
- Cao, Y. et al. Journal of Nanobiotechnology 21, 21 (2023). PMID: 36658555 — Application: WB, IF
- Yue, R. et al. ACS Appl. Mater. Interfaces 12, 32493–32502 (2020). PMID: 32573191 — Application: WB, IF
- Wang, S. et al. J. Neuroinflammation 16, 139 (2019). PMID: 31287006 — Application: WB, IF
- Li, Z. et al. Oxid Med Cell Longev 2022, 9035547 (2022). PMID: 36160707 — Application: WB, IF
- Qiu, L. et al. Food & Function 12, 10950–10966 (2021). PMID: 34647944 — Application: WB, IF
(Additional references available upon request.)
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