Thermo Fisher Scientific BACE1 Polyclonal Antibody

Applications

• Immunohistochemistry (Paraffin) [IHC(P)]: 2.5–5 µg/mL

Product Specifications

Product Specific Information

Percent identity with other species by BLAST analysis:
Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse, Rat, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Guinea pig (94%); Opossum (89%); Turkey, Chicken, Platypus (83%).

For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimer’s disease (AD). Early-onset familial AD can be associated with a “Swedish” mutation in the amyloid precursor protein (APP), which enhances cleavage by BACE1.
Therapeutic strategies targeting BACE1 and other APP-cleaving enzymes such as gamma-secretase have been explored. APP is first cleaved by beta-secretase, producing a soluble derivative and a membrane-anchored C99 fragment. The C99 fragment serves as substrate for gamma-secretase to generate amyloid-beta peptide, deposited in the brains of Alzheimer’s patients.
BACE1 (also known as Asp2) is a transmembrane aspartic protease that colocalizes with APP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.