Thermo Fisher Scientific PRKAR1B Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1–10 µg/mL

Immunocytochemistry (ICC/IF)

• Tested Dilution: 5 µg/mL

Product Specifications

Product Specific Information

• In Western blots, less than 1% cross-reactivity with recombinant human PKA RI alpha or rhPKA RII beta is observed; cross-reactivity with PKA RII alpha is unknown.
• Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism, and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme, resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, each representing specific gene products. Elevation of cAMP levels leads to activation of transcription via translocation of PKA to the nucleus, where it phosphorylates the transcription factor CREB on serine 133, enabling TFIIB binding to TBP1 and linking phospho-CREB to the pol II transcription initiation complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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