Thermo Fisher Scientific Phospho-GIT1 (Tyr554) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Dot blot (DB)

1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y554 of human GIT1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GIT1,uniProtId:Q9Y2X7-1,ncbiNodeId:9606,antigenRange:554,antigenLength:761,antigenImageFileName:PA5-12982_GIT1_Q9Y2X7-1_Rabbit.svg,antigenImageFileNamePDP:PA5-12982_GIT1_Q9Y2X7-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2109995

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

PA5-12982 was used in western blot to show induction of phophorylation of GIT1 on Y554 in lysates of human umbilical vein endothelial cells.

Target Information

GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. It may serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. It increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.