Thermo Fisher Scientific Phospho-GIT1 (Tyr554) Polyclonal Antibody
인간 GIT1의 Tyr554 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot 및 Dot blot에 사용 가능. 단백질 A 및 항원 친화 크로마토그래피로 정제. PBS buffer, 0.09% sodium azide 포함. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:200
Dot Blot (DB)
- Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y554 of human GIT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2109995 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
PA5-12982 was used in western blot to show induction of phosphorylation of GIT1 on Y554 in lysates of human umbilical vein endothelial cells.
Target Information
GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. It may serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion, and cytoskeletal organization. It increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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