Thermo Fisher Scientific RAP1A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 39-138 of human Rap1A (NP_0028751) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAP1A,uniProtId:P62834-1,ncbiNodeId:9606,antigenRange:39-138,antigenLength:184,antigenImageFileName:PA5-87540_RAP1A_P62834-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87540_RAP1A_P62834-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.78 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804237

Product Specific Information

Positive Samples: U-87MG, HL-60, Mouse lung, Mouse kidney, Rat kidney, Rat brain; Cellular Location: Cell junction, Cell membrane, Cytoplasm, Early endosome, Lipid-anchor, perinuclear region

Immunogen sequence: SYRKQVEVDC QQCMLEILDT AGTEQFTAMR DLYMKNGQGF ALVYSITAQS TFNDLQDLRE QILRVKDTED VPMILVGNKC DLEDERVVGK EQGQNLARQW

Target Information

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.