Thermo Fisher Scientific Mouse CXCL12 (SDF-1a) Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Mouse

Expression System

E. coli

Amino acid sequence

KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Molecular weight

7.9 kDa

Class

Recombinant

Type

Protein

Purity

>98% by SDS-PAGE

Endotoxin concentration

<0.1 ng/µg

Activity

ED50 = 50 - 100 ng/mL; determined by the dose-dependent chemotaxis of human PBMCs.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

Product Specific Information

Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. This lyophilized protein should be reconstituted in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in tissue culture grade buffered solutions with heat inactivated fetal calf serum or low endotoxin BSA.

Storage: Lyophilized human SDF-1 alpha should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted human SDF-1 alpha at < -20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.

Target Information

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.