Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 660
• Alexa Fluor Plus 488
• Alexa Fluor Plus 647
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 530, 555, 610-30S, 660-40S, 725, 800
• NovaFluor UV 765
• NovaFluor Violet 690, 800
• NovaFluor Yellow 810
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

Description:
The LN3 monoclonal antibody reacts with human MHC class II molecule HLA-DR, expressed on antigen-presenting cells (APCs) such as B cells, monocytes, macrophages, dendritic cells, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits, playing a crucial role in peptide presentation to CD4⁺ T lymphocytes.

Applications Reported:
LN3 antibody is reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage is 5 µL (0.01 µg) per 100 µL test volume. Applicable cell number: 10⁵–10⁸ cells/test.

Fluorochrome Information:
Alexa Fluor™ 700 emits at 723 nm and is excited with a red laser (633 nm). Recommended optical setup: 685 LP mirror and 710/20 filter.

• Excitation: 633–647 nm
• Emission: 723 nm
• Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

HLA-DR is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).

• The alpha chain gene (DRA) has 5 exons: Exon 1 encodes the leader peptide, exons 2–3 encode extracellular domains, and exon 4 encodes the transmembrane and cytoplasmic regions.
• DRA lacks polymorphisms in the peptide-binding region and pairs with DRB1, DRB3, DRB4, and DRB5.
• DRB1 alleles determine peptide-binding specificity, crucial for transplantation typing.
• Expressed mainly on antigen-presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.
• HLA-DR, DQ, and DP loci encode the main class II MHC molecules involved in antigen presentation to T-helper cells, initiating immune responses.

HLA and MHC antibodies are essential in Immunopeptidomics, aiding in neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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