Thermo Fisher Scientific NEDD4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 160-460 of human NEDD4 (NP_9406822) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NEDD4,uniProtId:P46934-1,ncbiNodeId:9606,antigenRange:160-460,antigenLength:1319,antigenImageFileName:PA5-87453_NEDD4_P46934-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87453_NEDD4_P46934-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804161

Product Specific Information

Immunogen sequence: GSYSSNGSDF GSCASITSGG SYTNSVISDS SSYTFPPSDD TFLGGNLPSD STSNRSVPNR NTTPCEIFSR STSTDPFVQD DLEHGLEIMK LPVSRNTKIP LKRYSSLVIF PRSPSTTRPT SPTSLCTLLS KGSYQTSHQF IISPSEIAHN EDGTSAKGFL STAVNGLRLS KTICTPGEVR DIRPLHRKGS LQKKIVLSNN TPRQTVCEKS SEGYSCVSVH FTQRKAATLD CETTNGDCKP EMSEIKLNSD SEYIKLMHRT SACLPSSQNV DCQININGEL ERPHSQMNKN HGILRRSISL G; Positive Samples: U-251MG, HeLa, Jurkat, A-549, SKOV3; Cellular Location: Cell membrane, Cytoplasm, Peripheral membrane protein

Target Information

The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.