Thermo Fisher Scientific GEMIN2 Monoclonal Antibody (OTI6G4), TrueMAB

Thermo Fisher Scientific GEMIN2 Monoclonal Antibody (OTI6G4), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
• For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and pre-mRNA splicing. SIP1 interacts directly with SMN and is required for formation of the SMN complex.
A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of SIP1 mRNA in motor neurons showed normal motor axons, while knockdown of SMN mRNA caused abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside the SMN complex.

Usage Note

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.