Thermo Fisher Scientific HLA-DR Monoclonal Antibody (TU36), PerCP

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

TU36

Immunogen

Human HLA-DR

Conjugate

PerCP PerCP PerCP

View additional formats

• APC
• FITC
• Pacific Blue
• Pacific Orange
• PE
• PE-Cyanine5.5
• PE-Texas Red
• Qdot 605
• Qdot 655
• Qdot 705
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS with sucrose, BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10401404

Product Specific Information

PerCP conjugates are excited by the 488 nm line of the argon-ion laser and have a large Stokes shift, providing minimal spectral overlap into the R-PE (phycoerythrin) channel.

This antibody recognizes the complex of the chains but not the individual chains.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Analyte Specific Reagent