Thermo Fisher Scientific ZAP-70 Monoclonal Antibody (1E7.2), PerCP

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1E7.2

Immunogen

GST-fusion protein of Human ZAP70, amino acids 282-307

Conjugate

PerCP PerCP PerCP

View additional formats

• Unconjugated
• Alexa Fluor 488
• Pacific Blue
• PE
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

Product Specific Information

PerCP conjugates are excited by the 488 nm line of the argon-ion laser and have a large Stokes shift, providing minimal spectral overlap into the R-PE (phycoerythrin) channel.

Target Information

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

Analyte Specific Reagent