Thermo Fisher Scientific p21 Polyclonal Antibody
Thermo Fisher Scientific의 p21 Polyclonal Antibody는 인간 및 생쥐 시료에 반응하며, WB·IHC·ELISA·IP 등 다양한 응용에 적합합니다. CDKN1A/p21CIP1 단백질을 타겟으로 하며, 액상 형태로 제공되고 -20°C에서 보관합니다. 종양 억제 단백질 연구에 활용됩니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific p21 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–164 of human CDKN1A/p21CIP1 (NP_000380.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914399 |
Product Specific Information
Positive test controls include MCF7, 293T, mouse large intestine, mouse spleen, and rat lung.
The target is usually found in the cytoplasm and nucleus.
Immunogen Sequence:
MSEPAGDVRQ NPCGSKACRR LFGPVDSEQL SRDCDALMAG CIQEARERWN FDFVTETPLE GDFAWERVRG LGLPKLYLPT GPRRGRDELG GGRRPGTSPA LLQGTAEEDH VDLSLSCTLV PRSGEQAEGS PGGPGDSQGR KRRQTSMTDF YHSKRRLIFS KRKP
Target Information
Protein p21 is a tumor suppressor protein that inhibits Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53.
It plays a crucial role in the cellular response to genotoxic stress and DNA damage repair.
- In the nucleus: binds and inhibits CDK1/CDK2, blocking G1→S and G2→M transitions.
- In the cytoplasm: exhibits anti-apoptotic effects by inhibiting caspase 3, ASK1, and JNK.
P21 has dual roles in carcinogenesis, functioning as both a tumor suppressor and oncogene depending on cellular context.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.