Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), NovaFluor Violet 690, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.6 µg/test

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet series
• eFluor 450, 506
• FITC
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright series
• NovaFluor Blue, UV, Violet, Yellow series
• Functional Grade
• Request custom conjugation

Product Specific Information

Description:
The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and by most peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation, proliferation, or apoptosis depending on assay conditions. It serves as a phenotypic marker for TCR beta-expressing T cells.

Applications Reported:
Flow cytometric analysis.

Applications Tested:
Tested on mouse thymocytes and splenocytes at ≤ 0.6 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Titrate for optimal performance.

Kit Components:
Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) enhances staining performance and should be added to antibody cocktails before mixing with cells (5 µL/sample).

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on engineered nucleic acid dye structures that deliver optimized fluorescent signatures with minimal spillover and spectral spread.

• Excitation: 408 nm
• Emission: 691 nm
• Laser: 405 nm (Violet)

Target Information

T cell receptors (TCRs) are essential for distinguishing foreign from self-peptides presented by MHC class II molecules, a key function in adaptive immunity. Dysregulated TCR recognition of self-peptides is linked to autoimmune diseases, while mutant self-peptides are associated with tumors. Superantigen-mediated TCR engagement can cause toxic shock syndrome. Autoantibodies to TCR V beta segments have been found in rheumatoid arthritis and SLE patients, potentially modulating TH1-mediated inflammatory responses.
Human T cells generally express TCR αβ with CD4 or CD8, while a smaller subset (double negative, DN) lacks both. γδ T cells, found mainly in epithelia, show limited TCR diversity and distinct antigen recognition, with subsets exhibiting antitumor and immunoregulatory functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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