Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), NovaFluor UV 765, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.4 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 661, 737
• Brilliant Violet 421
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510, 530, 610-70S, 660-120S, 725, 800
• NovaFluor Violet 690, 800
• NovaFluor Yellow 810
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and by most peripheral T cells. Crosslinking of the TCR complex with H57-597 can induce activation, proliferation, or apoptosis of T cells depending on assay conditions. It is commonly used as a phenotypic marker for TCR beta-expressing T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse thymocytes and splenocytes
  • Recommended ≤0.4 µg per test (100 µL final volume)
  • Cell number range: 10⁵–10⁸ cells/test
  • Optimize antibody concentration empirically

Each product contains one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample before combining antibodies with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton technology, based on nucleic acid dye structures engineered for optimized fluorescence with minimized spillover and spread.
Excitation: 358 nm Emission: 763 nm Laser: 349 nm (Ultraviolet)

Target Information

T cell receptors (TCRs) enable discrimination between self and foreign peptides presented by MHC class II molecules, critical for adaptive immunity. Recognition of self-peptides is linked to autoimmune diseases, while mutant peptides correlate with tumors. Superantigen engagement of TCRs can cause toxic shock syndrome. Autoantibodies to TCR V beta segments are found in rheumatoid arthritis and systemic lupus erythematosus, potentially modulating inflammatory responses.

Most human T cells express TCR alpha-beta along with CD4 or CD8. A smaller population lacks both markers (double negative), which can increase in autoimmune or immunodeficiency disorders. Gamma-delta T cells, primarily epithelial, exhibit distinct antigen recognition and may have antitumor and immunoregulatory functions.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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