Thermo Fisher Scientific Rabbit anti-Mouse IgG (H+L) Secondary Antibody, Rhodamine

Applications

Tested Dilution

Immunohistochemistry (IHC)

1:50 - 1:200

Immunocytochemistry (ICC/IF)

1:50 - 1:200

Flow Cytometry (Flow)

1:50 - 1:200

Immunoprecipitation (IP)

1:50 - 1:200

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Rhodamine Rhodamine Rhodamine

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488/host/Rabbit/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 555/host/Rabbit/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 647/host/Rabbit/)
• [AP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/AP/host/Rabbit/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Biotin/host/Rabbit/)
• [FITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/FITC/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/HRP/host/Rabbit/)
• [TRITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/TRITC/host/Rabbit/)
• Request custom conjugation

Excitation/Emission Max

573/591 nm View spectra

Form

Lyophilized

Concentration

1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_228428

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product # 31665 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 31665 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulin from other species.

Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Rhodamine Amax= 550 nm; Emax= 570 nm. Fluorophore/Protein Absorbance Ratio: A550/A280 = ~ 0.4 (lot-dependent).

Reconstitute with 1.1 mL of distilled water (1.5 mg/mL after restoration).

Country of Origin: USA

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.