Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Secondary Antibody, Rhodamine

Applications

Tested Dilution

Immunohistochemistry (IHC)

1:50 - 1:200

Immunocytochemistry (ICC/IF)

4 µg/mL

Flow Cytometry (Flow)

1:50 - 1:200

Immunoprecipitation (IP)

1:50 - 1:200

Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Rhodamine Rhodamine Rhodamine

View additional formats

• [AP](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/Biotin/host/Goat/)
• [FITC](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/HRP/host/Goat/)
• [NovaFluor Blue 510](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 510/host/Goat/)
• [NovaFluor Blue 555](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 555/host/Goat/)
• [NovaFluor Blue 585](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 585/host/Goat/)
• [NovaFluor Blue 610-70S](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 610-70S/host/Goat/)
• [NovaFluor Blue 660-120S](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 660-120S/host/Goat/)
• [NovaFluor Yellow 590](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 590/host/Goat/)
• [NovaFluor Yellow 610](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 610/host/Goat/)
• [NovaFluor Yellow 660](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 660/host/Goat/)
• [PE ](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/PE/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/TRITC/host/Goat/)
• [WesternDot 625](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/WesternDot 625/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

573/591 nm View spectra

Form

Lyophilized

Concentration

1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_228357

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product # 31680 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 31680 reacts with the heavy chains of rat IgG and with the light chains common to most rat immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, antibodies may cross-react with immunoglobulins from other species.

Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Rhodamine Amax= 550 nm; Emax= 570 nm. Fluorophore/Protein Absorbance Ratio: A550/A280 = 0.47 (lot-dependent).

Reconstitute with 1.5 mL of distilled water (1.5 mg/mL after restoration).

Country of Origin: USA

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.