Thermo Fisher Scientific Hemoglobin alpha Recombinant Rabbit Monoclonal Antibody (ARC1510)
Hemoglobin alpha 단백질을 검출하기 위한 Thermo Fisher Scientific의 재조합 토끼 단클론 항체입니다. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합하며 인간, 마우스, 랫트 반응성을 보입니다. 고순도 친화 크로마토그래피 정제, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1510 |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human Hemoglobin subunit alpha (HBA1, P69905) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849694 |
Product Specific Information
Immunogen sequence:
MVLSPADKTN VKAAWGKVGA HAGEYGAEAL ERMFLSFPTT KTYFPHFDLS HGSAQVKGHG KKVADALTNA VAHVDDMPNA LSALSDLHAH KLRVDPVNFK
Target Information
The human alpha globin gene cluster on chromosome 16 spans about 30 kb and includes seven loci:
5′- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3′.
The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical, differing slightly in untranslated regions and introns.
Two alpha and two beta chains form HbA, comprising ~97% of adult hemoglobin. Alpha chains also combine with delta chains to form HbA2, which along with HbF makes up the remaining ~3%.
Alpha thalassemias result from deletions or mutations of these alpha genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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