Thermo Fisher Scientific H3K27ac Monoclonal Antibody (2D7B3)
H3K27ac에 특이적인 마우스 모노클론 항체로, ELISA, IHC, 유세포분석에 사용 가능. Human 시료에 반응하며 Protein G로 정제된 액상 형태. 4°C 단기 보관, 장기 보관 시 -20°C 권장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D7B3 |
| Immunogen | Synthesized peptide of human HIST2H3C (AA: ATKAARK(Ac)SAPATGGV) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787383 |
Product Specific Information
MA5-31760 has been tested in indirect ELISA.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to either directly change the chromatin structure or allow for the binding of specific transcription factors.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several types of post-translational modifications that influence cellular processes, including methylation, acetylation, and phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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