Thermo Fisher Scientific Phospho-SIRT1 (Ser47) Polyclonal Antibody
Phospho-SIRT1 (Ser47) 항체로 SIRT1 단백질의 인산화 상태를 특이적으로 검출. Western blot, ICC/IF, Flow cytometry, IP 등 다양한 응용에 적합. Rabbit polyclonal IgG 형식으로 높은 특이성과 재현성 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | 1:100 | 1 publication |
| Immunoprecipitation (IP) | 1:25 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer47 of human SirT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 7.3 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10980703 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other sirtuin proteins.
Target Information
NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy.
SIRT1 modulates chromatin function through histone deacetylation and can alter histone and DNA methylation, leading to transcriptional repression.
It plays an essential role in skeletal muscle cell differentiation and mediates inhibitory effects on myoblast differentiation under low nutrient conditions involving AMPK and NAMPT.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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