Thermo Fisher Scientific Caspase-2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500- 1:2,000

-

ELISA (ELISA)

1:1,500-1:6,000

-

Immunoprecipitation (IP)

1:750

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Caspase 2,uniProtId:P55215-1,ncbiNodeId:10114,antigenRange:1,antigenLength:452,antigenImageFileName:600-401-412_Caspase_2_P55215-1_Rabbit.svg,antigenImageFileNamePDP:600-401-412_Caspase_2_P55215-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.44 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein. This antibody reacts with mouse and rat caspase-2, but not caspase-2 from human sources. Cross reactivity with caspase-2 from other sources has not been determined. Rat and mouse caspase-2 show 94% homology. There is no significant homology to the human form.

Target Information

Caspase 2 gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.