Thermo Fisher Scientific PYGM Polyclonal Antibody
Rabbit polyclonal antibody against human PYGM for IHC (paraffin) applications. Antigen affinity purified, supplied as liquid in PBS with 0.1% sodium azide. High species homology and validated for research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 20 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 14 amino acid peptide from C-terminus of human PYGM / Phosphorylase b |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550474 |
Product Specific Information
Percent identity with other species by BLAST analysis:
- Human, Gorilla, Gibbon, Baboon, Monkey: 100%
- Galago, Marmoset, Mouse, Rat, Hamster, Rabbit, Pig, Armadillo: 93%
- Dog, Panda, Horse: 86%
For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain.
Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle.
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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