Thermo Fisher Scientific PYGL Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 10 µg/mL
• 참고: Heat-induced antigen retrieval in pH 6.0 citrate buffer 권장
• 1차 항체 반응 후 biotinylated 2차 항체, alkaline phosphatase-streptavidin 및 chromogen 사용

Product Specifications

Product Specific Information

• Percent identity with other species by BLAST analysis:
  Human, Gorilla, Monkey, Dog (100%); Gibbon, Marmoset, Panda (90%); Mouse, Sheep, Hamster, Horse, Rabbit (80%)

Target Information

This gene encodes a homodimeric protein that catalyzes the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores.
This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15.
Activity of this enzyme is regulated by allosteric effectors and hormonal controls.
Humans have three glycogen phosphorylase genes encoding distinct isozymes primarily expressed in liver, brain, and muscle.
The liver isozyme serves the glycemic demands of the body, while the brain and muscle isozymes supply those tissues.
In glycogen storage disease type VI (Hers disease), mutations inhibit glycogen conversion to glucose, resulting in moderate hypoglycemia, mild ketosis, growth retardation, and hepatomegaly.
Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.