Thermo Fisher Scientific F(Ab`)2 Biotin Polyclonal Antibody, Cyanine5
Cyanine5로 표지된 Biotin F(ab`)2 폴리클로날 항체로 면역형광 및 유세포분석에 사용 가능. 염소 유래 IgG로 높은 특이성과 감도를 제공. 647/665 nm 파장 특성으로 다양한 형광 응용에 적합. 연구용으로만 사용.
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- 700-110-098
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:1,000
Flow Cytometry (Flow)
- Tested Dilution: 1:200–1:400
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Cyanine5 |
| Excitation / Emission Max | 647 / 665 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay Information:
Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotin conjugated Goat IgG, and biotin conjugated BSA. No reaction was observed against anti-Goat IgG F(c) or anti-Pepsin.
Target Information
Biotin is a water-soluble B complex vitamin (Vitamin B7). It is required by all organisms and synthesized only by bacteria, yeasts, molds, algae, and some plant species. Biotin functions in carbon dioxide incorporation into organic compounds and has a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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