Thermo Fisher Scientific Aldolase B Polyclonal Antibody
Rabbit polyclonal antibody recognizing Aldolase B in human, mouse, and rat samples. Validated for WB, IHC, and ICC applications. Suitable for detecting glycolytic enzyme isoforms. Supplied as liquid, affinity-purified, and stored at 4°C or -20°C.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 15–265 of Aldolase B (Uniprot ID: P05062) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.09 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552011 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, rat brain, Neuro2A, GL261, C8D30, PC-12
- Predicted reactivity: Mouse (96%), Rat (97%), Rabbit (98%), Chicken (84%), Sheep (90%), Bovine (91%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Fructose-1,6-bisphosphate aldolase is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have three aldolase isozymes (A, B, and C) distinguished by their electrophoretic and catalytic properties.
Aldolase A is predominant in muscle, Aldolase B in liver, kidney, and intestine, and Aldolase C in brain and nervous tissue.
Defects in the ALDOB gene cause hereditary fructose intolerance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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