Thermo Fisher Scientific PIK3C2A Monoclonal Antibody (OTI1A9), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The Class II PI3 kinases, including PIK3C2A, phosphorylate the 3 position of PI or PI4P. Though less studied than Class I PI3 kinases, Class II enzymes are linked to diverse receptor-mediated cellular processes such as insulin signaling, neuronal survival, and growth factor signaling.
PI3-kinases play roles in pathways regulating cell proliferation, oncogenic transformation, survival, migration, and intracellular trafficking.
PIK3C2A contains a lipid kinase catalytic domain and a C-terminal C2 domain, typical of Class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs mediating protein translocation to membranes and protein-protein interactions.
This protein’s PI3-kinase activity is not sensitive to nanomolar levels of wortmannin and may be activated by insulin, implicating it in integrin-dependent signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.