Thermo Fisher Scientific Cas9 Monoclonal Antibody (OTI5E5), TrueMAB
Cas9 단백질을 인식하는 고특이성 Mouse 단일클론 항체로, Western blot 및 Immunoprecipitation에 사용 가능. 인간 재조합 Cas9 단백질(aa 1-1166)을 면역원으로 제작. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunoprecipitation (IP)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5E5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–1166 of human CAS9 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering.
This system uses RNA to direct the nuclease to specific DNA sequences, enabling:
- Indel mutations
- Sequence replacements/insertions
- Large deletions or genomic rearrangements
- Upregulation of endogenous genes
- Alteration of histone modifications or DNA methylation
Cas9 variants include:
- SpCas9 (Streptococcus pyogenes)
- SaCas9 (Staphylococcus aureus)
SaCas9 advantages:
- Smaller size (1053 AA vs 1368 AA)
- High efficiency and specificity
- Recognizes NNGRRT PAM
- Suitable for all-in-one delivery systems using AAV vectors
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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