Thermo Fisher Scientific Dityrosine Monoclonal Antibody (10A6), FITC
Dityrosine 단백질 변형을 특이적으로 검출하는 FITC 결합 단일클론 항체. Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능. 마우스 IgG1 기반으로 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Dityrosine Monoclonal Antibody (10A6), FITC
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10A6 |
| Immunogen | Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932284 |
Product Specific Information
A 1:1,000 dilution of MA5-45830 was sufficient for detection of dityrosine in 1 µg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for dityrosine-modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
Target Information
Dityrosine may be a natural component of protein structure, a product of environmental stress, or a product of in vitro protein modification.
It is involved in diverse reactions that produce tyrosyl radicals, which may couple to yield dityrosine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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