Thermo Fisher Scientific Phospho-ULK1 (Ser317) Polyclonal Antibody
ULK1 단백질의 Ser317 인산화 형태를 특이적으로 검출하는 rabbit polyclonal antibody. Western blot, IHC, ICC/IF에 사용 가능. 자가포식 관련 연구에 적합하며, 고순도의 액상 형태로 제공됨. -20°C 보관.
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- PA5104556
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- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ULK1 (Accession O75385), corresponding to amino acid residues around phosphorylated Ser317. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816031 |
Product Specific Information
This antibody detects endogenous levels of ULK1 only when phosphorylated at Ser317.
Target Information
ULK1 is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy, a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as rapamycin-induced autophagy, consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13, which is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and for ULK1 protein stability. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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