Thermo Fisher Scientific NSE Polyclonal Antibody
Neuron Specific Enolase(NSE)를 검출하는 Thermo Fisher Scientific의 폴리클로날 항체로, 인간·마우스·랫트 반응성. WB, IHC, ICC/IF에 사용 가능하며, 신경세포 및 신경내분비 세포 식별에 적합. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:3,000–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full-length human NSE expressed in and purified from E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1077200 |
Product Specific Information
PA1-10010 can be used to trace NSE and to identify neuronal cells in cell culture and sectioned material.
Target Information
Neuron Specific Enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis.
NSE is highly stable in biological fluids and diffuses into cerebrospinal fluid (CSF) when neuronal membranes are injured. It is one of three mammalian enolases—alpha (ENO1), beta (ENO3), and gamma (ENO2)—with gamma-subunit expressed primarily in neurons and neuroendocrine cells.
Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. NSE antibodies are useful for identifying neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells.
Release of NSE from damaged neurons into CSF and blood serves as a biomarker of neuronal injury. Clinically, NSE is a sensitive marker of neuronal damage in disorders such as stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Elevated plasma NSE levels are also associated with neuroblastoma and small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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