Thermo Fisher Scientific MBP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

-

Immunohistochemistry (IHC)

1:10,000-1:20,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:2,000-1:5,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Published species

Mouse, Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Full length purified myelin basic protein isolated from cow nerve if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MBP,uniProtId:P02687-1,ncbiNodeId:9913,antigenRange:1-169,antigenLength:169,antigenImageFileName:PA1-10008_MBP_P02687-1_Chicken.svg,antigenImageFileNamePDP:PA1-10008_MBP_P02687-1_Chicken_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1077024

Product Specific Information

In Western blot using rat spinal cord whole tissue homogenates, this antibody detects all isotypes of MPB, resulting in bands at ~21.5kDa, 18.5kDa, 17kDa and 14kDa. |Stable at 4°C for at least one year.

Target Information

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called Golli-MBP) that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.