Thermo Fisher Scientific Rhodopsin Monoclonal Antibody (1D4), PerCP
Rhodopsin 단백질 검출용 PerCP 결합 단클론 항체로, Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. 사람 및 생쥐 반응성, 높은 특이성과 민감도 제공. 단백질 G 정제, 4°C 보관.
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Thermo Fisher Scientific Rhodopsin Monoclonal Antibody (1D4), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D4 |
| Immunogen | Bovine Rhodopsin |
| Conjugate | PerCP |
| Excitation/Emission Max | 482/675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931703 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45249 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 40 kDa. Recognizes Rhodopsin (native and recombinant forms).
No known reactivity to other proteins.
Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
Target Information
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision.
Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal.
This receptor is coupled to the activation of phospholipase C.
Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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