Thermo Fisher Scientific Rhodopsin Monoclonal Antibody (1D4), FITC
Rhodopsin 단백질을 특이적으로 인식하는 1D4 클론의 FITC 결합 단일클론 항체. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. 사람 및 생쥐 시료에 반응하며, 498/517 nm 형광 특성. 4°C 암소 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D4 |
| Immunogen | Bovine Rhodopsin |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931702 |
Additional Formats
- APC: MA5-45247
- PerCP: MA5-45249
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45248 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 40 kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins.
Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
Target Information
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision.
Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C.
Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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