Thermo Fisher Scientific SP1 Monoclonal Antibody (GT2574)
Human SP1 단백질을 인식하는 단일클론 항체로 Western blot, IHC, ICC/IF 등에 사용 가능. Mouse IgG2a 형식이며 Protein A로 정제된 액상 제품. 4°C 단기 보관, -20°C 장기 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT2574 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SP1 (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735369 |
Product Specific Information
- Positive Control: THP-1
- Predicted Reactivity: Mouse (96%), Rat (99%), Rhesus Monkey (99%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in cell growth, apoptosis, differentiation, and immune responses. SP1 activity is highly regulated by post-translational modifications such as phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation. It also binds the PDGFR-alpha G-box promoter and may modulate cellular responses to DNA damage. SP1 is implicated in chromatin remodeling and plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter, as well as in the regulation of FE65 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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