Thermo Fisher Scientific TDG Monoclonal Antibody (GT1142)
Human TDG 단백질을 인식하는 Mouse IgG2b 단일클론 항체로 Western blot 및 Immunocytochemistry에 사용 가능. Recombinant TDG 중심 영역을 면역원으로 제작. Protein A 정제, PBS buffer, 무보존제. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT1142 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human TDG (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735389 |
Product Specific Information
- Positive Control: human TDG-transfected 293T cells
- Predicted Reactivity: Mouse (100%), Rat (99%), Xenopus laevis (92%), Chicken (95%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly before opening the vial.
Target Information
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family.
Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine.
With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine.
This enzyme plays a central role in cellular defense against genetic mutation caused by spontaneous deamination of 5-methylcytosine and cytosine.
This gene may have a pseudogene in the p arm of chromosome 12.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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