카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Takara · Takara Micrococcal Nuclease
Applications
- Digestion of nucleotides included in crude cell extract
- Digestion of chromatin for nucleosome preparation
- Chromatin immunoprecipitation (ChIP)
Supplied Buffer
10X Micrococcal Nuclease Buffer: 200 mM Tris-HCl (pH 8.0), 50 mM NaCl, 25 mM CaCl₂
Formulation
| Component | Concentration / Composition |
|---|---|
| Tris-HCl | 50 mM (pH 8.0) |
| 2-Mercaptoethanol | 10 mM |
| NaCl | 50 mM |
| Glycerol | 50% |
Product Citations
- Lefevre, P. & Bonifer, C. (2006). Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease. Methods Mol. Biol. 325:315–325.
- Heins, J.N. et al. (1966). Procedures Nucl. Acids Res. 1:79.
- Pelham, H.R. & Jackson, R.J. (1976). An Efficient mRNA-Dependent Translation System from Reticulocyte Lysates. Eur. J. Biochem. 67:247–256.
- Telford, D.J. & Stewart, B.W. (1989). Micrococcal nuclease: Its specificity and use for chromatin analysis. Int. J. Biochem. 21:127–137.
Additional Product Information
Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Additional product, intellectual property, and restricted use information is available at takarabio.com.
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