Takara Micrococcal Nuclease

More Information

Applications

• Digestion of nucleotides included in crude cell extract
• Digestion of chromatin for nucleosome preparation
• Chromatin immunoprecipitation (ChIP)

Supplied buffer

10X Micrococcal Nuclease [200 mM Tris-HCl (pH 8.0), 50 mM NaCl, 25 mM CaCl2]

Form

50 mM Tris-HCl (pH 8.0), 10 mM 2-mercaptoethanol, 50 mM NaCl, 50% glycerol

Product citations

Lefevre, P. & Bonifer, C. Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease. Methods Mol. Biol. 325:315–325 (2006).

Heins, J.N. et al. Procedures Nucl. Acids Res. 1:79 (1966).

Pelham, H.R. & Jackson, R.J. An Efficient mRNA-Dependent Translation System from Reticulocyte Lysates. Eur. J. Biochem. 67:247–256 (1976).

Telford, D.J. & Stewart, B.W. Micrococcal nuclease: Its specificity and use for chromatin analysis. Int. J. Biochem. 21:127–137 (1989).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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