Takara Micrococcal Nuclease

Applications

• Digestion of nucleotides included in crude cell extract
• Digestion of chromatin for nucleosome preparation
• Chromatin immunoprecipitation (ChIP)

Supplied Buffer

10X Micrococcal Nuclease Buffer: 200 mM Tris-HCl (pH 8.0), 50 mM NaCl, 25 mM CaCl₂

Formulation

Product Citations

• Lefevre, P. & Bonifer, C. (2006). Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease. Methods Mol. Biol. 325:315–325.
• Heins, J.N. et al. (1966). Procedures Nucl. Acids Res. 1:79.
• Pelham, H.R. & Jackson, R.J. (1976). An Efficient mRNA-Dependent Translation System from Reticulocyte Lysates. Eur. J. Biochem. 67:247–256.
• Telford, D.J. & Stewart, B.W. (1989). Micrococcal nuclease: Its specificity and use for chromatin analysis. Int. J. Biochem. 21:127–137.

Additional Product Information

Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliates. Certain trademarks may not be registered in all jurisdictions.
Additional product, intellectual property, and restricted use information is available at takarabio.com.