Thermo Fisher Scientific IL-12/IL-23 p40 Monoclonal Antibody (C17.8), Functional Grade, eBioscience
IL-12/IL-23 p40 단일클론 항체(C17.8)는 IL-12 및 IL-23의 생물학적 활성을 중화하는 기능 등급 항체입니다. 다양한 면역학적 분석(WB, ELISA, Flow Cytometry, IP, Neutralization)에 적합하며, 고순도·저엔도톡신 수준으로 제조되었습니다. 연구용으로 사용됩니다.
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Thermo Fisher Scientific IL-12/IL-23 p40 Monoclonal Antibody (C17.8), Functional Grade, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Flow Cytometry (Flow) | Assay-Dependent | 12 publications |
| ELISA | - | 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | Assay-Dependent | 8 publications |
| Functional Assay | Assay-Dependent | 8 publications |
| T-Cell Activation (TCA) | - | 1 publication |
| In vitro Assay (IV) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C17.8 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2866234 |
Additional Formats:
- Alexa Fluor 488
- Biotin
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine7
- PerCP-Cyanine5.5
- Custom conjugation available
Product Specific Information
The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23, neutralizing their bioactivity.
For specific neutralization of IL-23 only, refer to clone G23-8 (anti-mouse IL-23 p19).
IL-12
- 70 kDa heterodimeric cytokine composed of p40 and p35 chains
- Secreted by activated monocytes, macrophages, and dendritic cells
IL-23
- Heterodimer composed of disulfide-linked p19 and p40 subunits
- Expressed by activated dendritic cells
Applications Reported: IP, Western blot, ELISA, neutralization, and intracellular flow cytometry.
Applications Tested: Verified by LAL assay for low endotoxin levels; tested for ELISA detection and bioassay neutralization of IL-12 p70 and IL-23.
At 62.0 ng/mL, the antibody inhibits by 50% the biological effects of 1 ng/mL mouse IL-12 p70 or IL-23 in bioassays.
Detailed protocols are available in the BestProtocols® Cytokine Bioassays Guide.
Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).
Target Information
IL-12
Produced by dendritic cells, neutrophils, macrophages, and lymphoblastoid cells upon antigenic stimulation.
Encodes IL-12A (p35) and IL-12B (p40).
Promotes leukocyte migration to tumors and induces ischemic necrosis.
IL-23
Shares IL-12B subunit with IL-12 and includes IL-23A (p19).
Stimulates memory T-cells and supports Th17 cell functions, enhancing neutrophil chemokine and pro-inflammatory cytokine production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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