Thermo Fisher Scientific PKC zeta Polyclonal Antibody
PKC zeta 단백질 인식용 토끼 다클론 항체로, Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능. Mouse 반응성, Rat에서 검증됨. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공. 세포 성장 및 신호전달 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 | – |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | – |
| Immunoprecipitation (IP) | Assay-dependent | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from the middle region of the catalytic domain of the mouse protein kinase C, zeta isoform (PKC-zeta) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533357 |
Target Information
The PKC family of serine/threonine kinases, including PRKCZ (PKC zeta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. PKC zeta plays a key regulatory role in various cellular functions including cell growth and differentiation, gene expression, hormone secretion, and membrane function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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